Stem Cell Biology

Embryonic Stem cell culture,Differentiation to desired cell types.

Need of cellular reprogramming. History of induced pluripotent stem cells. Generation of iPSCs, Maintenance and in vitrodifferentiation.

Case study-

In vitro differentiation – In Vitro Differentiation of Embryonic and Adult Stem Cells into Hepatocytes.

Understanding Trans-differentiation; Neuronal trans-differentiation from Mesenchymal Stem cells.

What are Cancer Stem cells? Types of Cancer Stem cells discovered till date

Molecular markers of Cancer stem cells. Mechanisms ruling cancer stem cells behaviour and responses. Implications of cancer stem cell research. Cancer stem cell analysis by Aldefluor assay.

Lineage tracing- Cre-  LoxP System, Flowcytometry with focus on stem cells, CRISPER, iRNA (Interference RNA technology) and microRNA, Gene expression analysis tools, Blastocyst injection technique for ESC fabrications

Introduction of regenerative medicine. Cord blood banking, stem cell banks.

Neurodegenerative Disorders and Spinal cord injury, Diabetes: Stem Cells as insulin producing β cells, Reviving skin in conditions of burns and skin ulcers, Stem cells in restoring disrupted musculo-skeletal abilities.

Robert LanzaEssentials of Stem Cell Biology.
FreshneyCulture of Animal Cell, A manual of Basic Technique.

A bivalent chromatin structure marks key developmental genes in embryonic stem cells.
Bradley E. Bernstein, Tarjei S. Mikkelsen, XiaohuiXie, Michael Kamal, Dana J. Huebert, James Cuff, Ben Fry, Alex Meissner, Marius Wernig, Kathrin Plath, Rudolf Jaenisch, Alexandre Wagschal, Robert Feil, Stuart L. Schreiber and Eric S. Lander,  Cell 125, 315–326, April 21, 2006
https://www.ncbi.nlm.nih.gov/pubmed/16630819

Regulation of Stem Cell Pluripotency and Differentiation Involves a Mutual Regulatory Circuit of the Nanog, OCT4, and SOX2 Pluripotency Transcription Factors With Polycomb Repressive Complexes and Stem Cell microRNAs
VasundhraKashyap, Naira C. Rezende, Kymora B. Scotland, Sebastian M. Shaffer,
Jenny Liao Persson,6 Lorraine J. Gudas,1 and Nigel P. Mongan, Stem Cells abd Development,
Volume 18, Number 7, 2009. DOI: 10.1089/scd.2009.0113

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135180/

The stem cell niche: theme and variations
Benjamin Ohlstein, Toshie Kai, Eva Decotto and Allan Spradling

DOI 10.1016/j.ceb.2004.09.003 Current Opinion in Cell Biology 2004, 16:693–699
https://www.google.co.in/url?q=http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%2520601/pdf%2520files/stemcell%2520niche%2520review.pdf&sa=U&ved=0ahUKEwixtIbT67bRAhVKsY8KHafKBUYQFggdMAE&sig2=Howeg8cRyqpvJF8uSlcZQg&usg=AFQjCNEL8gWhS1IkTWSMYJCqytLTC9W4Gw

Self-renewal and differentiation capacity of young and aged stem cells
Valerie D. Roobrouck, Fernando Ulloa-Montoya, Catherine M. Verfaillie, Experimental Cell Research, 314(2008)1937–1944
https://www.google.co.in/url?q=https://lirias.kuleuven.be/bitstream/123456789/204639/2/Roobrouck%2520-%2520Experimental%2520Cell%2520Research%2520-%25202008.pdf&sa=U&ved=0ahUKEwjKz-Gp67bRAhUIpo8KHZZSBjEQFggbMAA&sig2=Axu2dHiyaMlCzHxPmxDkbQ&usg=AFQjCNGpDyK2N33u3nlq_NkiocnPlDRpaw

Plasticity of Adult Stem Cells
Amy J. Wagers1, Irving L. Weissman Cell, Vol. 116, 639–648, March 5, 2004.
http://www.sciencedirect.com/science/article/pii/S0092867404002089
 
 
In Vitro Differentiation of Embryonic and Adult Stem Cells intoHepatocytes: State of the Art,
Sarah Snykers, Joery De Kock, Vera Rogiers, Tamara Vanhaecke, November 21, 2008; Stem Cell Express, December 4, 2008
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729674/

Embryonic stem cell lines from humanblastocysts: somatic differentiation in vitro
Benjamin E. Reubinoff1,2, Martin F. Pera1*, Chui-Yee Fong3, Alan Trounson1, and AriffBongso Nature Biotechnology vol 18 april 2000.
https://www.researchgate.net/publication/12566888_Embryonic_stem_cell_lines_from_human_blastocysts_somatic_differentiation_in_vitro_Nat_Biotechnol_184399-404_see_commenterratum_appears_in_Nat_Biotechnol_2000_May185559
 
Neural trans-differentiation of mesenchymal stem cells – a critical review
Christina Krabbe, Jens Zimmer and Morten Meyer APMIS 113: 831–44, 2005
Induced pluripotent stem cells from a spinal muscular atrophy patient
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2659408

Methods for Cancer Stem Cell Detection and Isolation.
Virginia Tirino, Vincenzo Desiderio, Francesca Paino,GianpaoloPapaccio, and Mario De Rosa, Somatic Stem Cells: Methods and Protocols, Methods in Molecular Biology, vol. 879
https://www.researchgate.net/publication/225049966_Methods_for_Cancer_Stem_Cell_Detection_and_Isolation

Cancer Stem Cells
Wei Guo, Joseph L. Lasky IIIand Hong Wu, Pediatric Research, Vol. 59, No. 4, Pt 2, 2006
http://www.brainlife.org/fulltext/2006/guo_w060401.pdf

Theory and Practice of Lineage Tracing

Ya-Chieh Hsu, Stem Cells 2015;33:3197–3204
https://www.ncbi.nlm.nih.gov/pubmed/26284340

Lineage Tracing
Kai Kretzschmar1 and Fiona M. Watt Cell 148, January 20, 2012
http://www.sciencedirect.com/science/article/pii/S0092867412000037

Detection of Hematopoietic Stem Cells by Flow Cytometry
Kuanyin K. Linand Magaret A. Goodell, Methods in Cell Biology, Vol 103
https://www.ncbi.nlm.nih.gov/pubmed/21722798

Critical Role of Flow Cytometry in EvaluatingPeripheral Blood Hematopoietic Stem Cell Grafts
Michael Keeney,1 Jan W. Gratama,2 and D. Robert Sutherland3 Cytometry Part A 58A:72–75 (2004)
https://www.ncbi.nlm.nih.gov/pubmed/14994224
Genome engineering using the CRISPR-Cas9 system

F Ann Ran, Patrick D Hsu, Jason Wright, VineetaAgarwala, David AScott and Feng Zhang Nat Protoc. 2013 November; 8(11): 2281–2308
https://www.ncbi.nlm.nih.gov/pubmed/25408407

A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal
Guang Hu,Jonghwan Kim, Qikai Xu, YumeiLeng, Stuart H. Orkin, and Stephen J. Elledge Genes & Development 23:837–848
http://genesdev.cshlp.org/content/23/7/837.full.pdf+html
 
RNAi in Stem Cells: Current Status and Future Perspectives
Gang-Ming Zou, Methods in Molecular Biology 650,DOI 10.1007/978-1-60761-769-3_1
https://www.ncbi.nlm.nih.gov/pubmed/20686939
Gene Expression Profiling of Embryonic Stem Cells Leads to Greater Understandingof Pluripotency and Early Developmental Events
Raj R. Rao and Steven L. SticeBiology of Reproduction,71, 1772–1778 (2004)
https://www.ncbi.nlm.nih.gov/pubmed/15140800
Generation of Gene Knockout Mice by ES Cell Microinjection
Glenn Longenecker, and Ashok B Kulkarni,CurrProtoc Cell Biol. 2009 September; Chapter: Unit–19.1436
https://www.ncbi.nlm.nih.gov/pubmed/15140800

Stem Cell Technology for Neurodegenerative Diseases.

1. Simon Lunn, Stacey A. Sakowski, JungukHurand Eva L. Feldman, Ann Neurol. 2011 September; 70(3): 353–361

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177143/pdf/nihms-297749.pdf

In vivo reprogramming of adult pancreatic exocrine cells to beta-cells (nature).
Qiao Zhou, Juliana Brown, Andrew Kanarek, JayarajRajagopal& Douglas A. Melton
Vol 455, 2October 2008, doi:10.1038/nature07314
http://web.slas.ac.cn/upload/20081107-2.pdf
Extensive tissue-regenerative capacity of neonatal human keratinocyte stem cells and their progeny.

Amy Li, Normand Pouliot, Richard Redvers, and Pritinder Kaur, J. Clin. Invest. 113:390–400 (2004)
https://www.ncbi.nlm.nih.gov/pubmed/14755336

Morphogenesis and renewal of hair follicles from adult multipotent stem cells.
Hideo Oshima,Ariane Rochat,Ce´cileKedzia, Koji Kobayashi, and Yann Barrandon  Cell, Vol. 104, 233–245, January 26, 2001
https://www.ncbi.nlm.nih.gov/pubmed/11207364

Mesenchymal Stem Cells: Cell-Based Reconstructive Therapy in Orthopedics.
Arnold I. Caplan, Tissue engineering, Volume 11, Number 7/8, 2005
https://phpsistemas.dti.ufv.br/noticia/files/anexos/php0emhxf_5654.pdf