It offers interesting new applications, such as for the quantification and genotyping of pathogens, gene expression, methylated DNA and microRNA analysis, validation of microarray data, allelic discrimination and genotyping (analysis of SNPs and microsatellites, identification of chromosomal alterations), validation of drug therapy efficacy, forensic studies and quantification of gene expression in genetically modified organisms (GMOs).
Real Time PCR technology merges the polymerase chain reaction chemistry with the use of fluorescent reporter molecules in order to monitor the production of amplification products during each cycle of the PCR reaction. In addition to quantification experiments, qPCR can be used for the analysis of copy number variations, DNA methylation, and genomic variants.
This exciting technology has enabled the shift of molecular diagnostics toward a high-throughput, automated technology with lower turnaround times. Thus Keeping in mind, the requirements of today’s research and industry, we maintain state of the art instrumental setup with a competent team where customized qPCR projects always help students to find new endeavor in global world and stood confidently for preparing for interviews or applying for PhD across the world. There is huge opportunities for well trained qPCR person for gene expression studies in Genomics, Bio-pharmaceuticals, Vaccine production industries, Human and Animal diagnostic centres, Bioprocessing units and many more…
A combination of lectures and hands on laboratory experience in using real-time PCR methods. Gain experience using DNA binding dyes and hydrolysis probe chemistries as well as real-time PCR. Lectures include an overview of gene expression, assay design and optimization, analysis of results, applications of real-time PCR technology, and more