flowcytrometricstudent

Introduction

Recent approaches to the study of membrane proteins have involved detecting specific proteins by sodium dodecyl sulfate (SDS)-gel electrophoresis, immunoprecipitation (l), purification of individual proteins and the use of fluorescent probes and fluorescence microscopy which is the time-consuming and laborious techniques. Only the latter technique permits following the fate of an individual membrane protein on single cells, but it is qualitative and insensitive. The flow cytometer and the fluorescence-activated cell sorter (FACS) are widely used analysis tools in biomedical research and clinical diagnostics. Flow cytometry provides a rapid and detailed analysis of cellular populations, attested as a valuable tool for diagnosis by cell counting, cell sorting,

biomarker detection and protein engineering, by defining different cell types in heterogeneous cell populations, assessing the purity of isolated subpopulations and analyzing cell size and volume along with relative fluorescence intensity. It is predominantly used to measure fluorescence intensity produced by fluorescent-labeled antibodies detecting proteins, or ligands that bind to specific cell-associated molecules such as Propidium Iodide binding to DNA.

The unique capability to analyze a large number of single cells for several parameters simultaneously, flow cytometry has changed our understanding of the behavior of cells in culture and of the population dynamics even of clonal populations. The fully hands on training in flow cytometry provided by IABRD gives the  platform for clinicians, basic researchers to get chance to apply in Bio-pharmaceuticals, Research institutes and R n D departments working in fields of Cell Biology, Cell Health, Immunology, Stem cells, Biosynthesis of active ingradients, Drug discovery programmes for cell cycle studies, gene expression levels, intracellular cytokine measurement, vaccine analysis, phagocytosis, and much more.

Besides its applications in basic biomedical research, flow cytometry can be utilised in hematological diagnosis to monitor the progression of diseases such as leukemia and AIDS. However, Flow cytometry has been developed to rapidly enumerate microorganisms; to distinguish between viable, metabolically active and dead cells, which is of great importance in food development and food spoilage; and to detect specific pathogenic microorganisms by conjugating antibodies with fluorochromes, which is of great use in the food industry.

Thus by appearing for this course you will gain not only expertise and know-how, but opens wide array of opportunities in different fields of life sciences.

Course I: FLOW CYTOMETRY Training Workshop

Course code: FLOWS

Duration: 1 day

Batch Timings : 4-6 pm Monday to Friday

Batch Size: 10 students

Course Introduction:
One day training program that provides training information or lecture and demonstration about Flow Cytometry basics like principle, instrumentation, applications and 2 hour demonstration in Lab by experts on sample acquisition and analysis. (Batch of 10 students only, Certificate of Attendance of Flow Cytometry workshop will be provided.)

Course Fees: 2000 /-

Name of the Topic

Content covered

Flow Cytometry: Basic Principle

Course Introduction

Principle and working of Instrument

Sample analysis

Sample Preparation and Analysis

Detection of Cellular Characteristics

a. Intrinsic Cellular Characteristics: Cell Size   and Granularity

b.  Extrinsic Cellular Characteristics: Surface Antigens

c. Calculating total Cell Counts

Result Interpretation

Cytogram for Cellular Scatter:

  • Intrinsic and Extrinsic Cell Characteristics
  • Gating: Selecting the Population of Interest
Recap Session

Questions and answers Session and Certificate distribution

Course II: Flow Cytometry Advance Hands-on Training

Course Code: FLOART

Duration: 5 days

Batch Timings : 4-6 pm Monday to Friday

Batch Size: 5 students

New batch starts: 1st and 3rd Monday every month

Course Information:
Three  days workshop is designed to provide lectures and hands-on training on Flow Cytometry. The course involves Basics of Flow Cytometry like Principle, Instrumentation, Applications, Choosing filters, Antibody selection, Compensation techniques, Cell Gating techniques, Trouble shooting and analysis on data on Third party Software FLOWJO. 3 days of hands-on demonstration with samples, involves sample preparation techniques, sample acquisition, using a single color and multiple color analysis in same cell population using flurophore tagged antibodies. Certification of completion of Advance Hands on Training in Flow Cytometry will be provided at the end of training.

Course Fees: 6500/-

Course Overview:

Name of the Topic

Content covered

Flow Cytometry: Basic Principle

Course Introduction

Principle and working of Instrument

Sample analysis:

Sample Preparation and Analysis

Detection of Cellular Characteristics

d. Intrinsic Cellular Characteristics: Cell Size   and Granularity

e. Extrinsic Cellular Characteristics: Surface Antigens

f. Calculating total Cell Counts

Result Interpretation:

Cytogram for Cellular Scatter: Intrinsic and Extrinsic Cell Characteristics

Gating: Selecting the Population of Interest

Case Study #1: To count and differentiate total blood lymphocyte/ T cell and B cell/ T cell population

Identification of these cell types on the basis of their associated Surface Markers

Calculating total Cell Counts

Review of Flow Cytometry Principles

Mock test on course topic

Short Presentation by trainees

Question and Answering session followed by Expert’s comment

Ongoing Projects:

Anti-cancer Drug Development: Aspects for sustainable Drug Development.

Despite major research in oncology R&D, the translation of research advances into medicines that surely improve the treatment of many cancer which remains frustratingly slow. The key challenges in anti-cancer drug development and mechanisms of drug molecule are meagerly studied. The flow cytometric analysis is proved to be the best option in recent time to understand such mechanisms on cell cycle studies of cancerous cell line. Hence; IABRD has taken initiative to decipher effect and mechanisms of drug molecules in cancer cell lines at cellular level.