Cancer Biology

Cancer describes an enormous spectrum of diseases that all originate from uncontrolled cellular growth. Broadly divided into benign tumors (unable to metastasize) or malignant tumors (able to invade normal tissues), cancers are further defined and classified by their cell type, tissue, or organ of origin. Cancer remains the number two cause of death in the U.S., second only to heart disease, and the Intramural Research Program (IRP) is committed to advancing research, building expertise, and leveraging resources to address this global challenge. Our scientists are constantly learning more about the causes of cancer, and developing new and better ways to prevent, detect, and treat it.

Work Flow

  1. Tumor
  2. Disintegration and single cell
  3. suspension
  4. preparation

Cancer biology Research

Instrument Details

gentleMACS™ Dissociator from Miltenyi Biotech

The gentleMACS™ Dissociator is a benchtop instrument for the semi-automated dissociation of tissues into single-cell suspensions or thorough homogenates. A single sample or two samples in parallel can be processed. Two types of unique gentleMACS tubes used with the instrument enable the time-saving and easy dissociation or homogenization of tissues in a closed system.

Consumables

All types of cell dissociator kits including

  • Mouse or human tumor
  • Mouse or rat neonatal heart
  • Neural Tissue
  • Mouse spleen, lung, lamina propria, muscle, epidermis or liver
  • Mouse or human skin and more

Flow Cytometer/Magnetic cell sorting system

Attune NxT Acoustic Focusing Cytometer, which is an easy-to-use instrument designed to detect cell size and granularity, as well as fluorescence expression of labeled antibodies, proteins, and functional reagents. The innovative design of the instrument results in precise data and acquisition speeds up to 10X faster than other flow cytometers. The design also eliminates lengthy sample preparation procedures, reduces the time to analyze a file, allows small or limited samples to be analyzed, and minimizes variability in measurements, leading to better resolution of populations. It is ideal for immunophenotyping and signaling studies, cell cycle analysis, detection of rare events, stem cell analysis, cancer and apoptosis studies, and microbiological assays.

Consumables

  • DNA staining dye for ploidy level detection
  • Antibodies for tumor cells CTCs, CSCs, Aldiflour Assay, Taqman Assay
  • Shut down solutions
  • Calibration Beads
  • Focussing fluid
  • Antibodies
  • Wash Solution
  • Trypan Blue
  • Cell fixation Buffer
  • Media (DMEM /RPMI/ PBS /HBSS /M199 /DPBS /SF 900
  •  Sera ( FBS, FCS, Horse Serum, Human Serum)
  • Cell Dissociation Reagents (Trypsine /TripLE Express / TripLE select / Accutase
  • Annexin PI / MTT
  • ROS dyes (CellTrace)
  • Cell Proliferation dye( CFSE / Blue dye )

Biosafety cabinet

Class II, Type A2 Biological Safety Cabinets offer personnel, product, and environmental protection to obtain optimum control over product quality while reducing the potential for exposure of both product and personnel to airborne biological or particulate chemical agents in low to moderate risk-hazard research and drug preparation or product operations.

Consumables

  • Culture plates (6/12/24/ 48/96 well )
  • Media (DMEM /RPMI/ PBS /HBSS /M199 /DPBS /SF 900)
  • Antbiotics and Antimycotics
  • Syringe Filters
  • Growth factors and Serums
  • Cell culture flasks (T 25/75/225/525)
  • Serological Pipettes
  • Cell Strainers

Inverted Microscope

EVOS M5000 Imaging System
Key Advantages

 

    1. Easy installation; no maintenance, assembly, alignment or calibration
    2. User defined four-color fluorescence, transmitted light and color applications
    3. Autofocus, Z-stack capability, time-lapse imaging and single-click multi-channel capture
    4. On-board software for fast acquisition and analysis, including confluency measurements and automated cell counting
    5. Access to Thermo Fisher Cloud imaging app

Consumables

  • Media (DMEM /RPMI/ PBS /HBSS /M199 /DPBS /SF 900
  • Sera ( FBS, FCS, Horse Serum, Human Serum)
  • Cell Dissociation Reagents (Trypsine /TripLE Express / TripLE select / Accutase
  • Antibodies
  • Annexin PI / MTT
  • ROS dyes (CellTrace)
  • CFSE dye
  • Trypan Blue
  • Cell fixation Buffer
  • Chamber Slides / Heamocytometer
  • 6/12/24/ 48/96 well tissue culture treated plates
  • Tissue culture flasks T-25/T75/T275
  • Serological Pipettes 5ml/ 10ml
  • Cell scraper

Inverted Microscope

EVOS M5000 Imaging System
Key Advantages

    1. Easy installation; no maintenance, assembly, alignment or calibration
    2. User defined four-color fluorescence, transmitted light and color applications
    3. Autofocus, Z-stack capability, time-lapse imaging and single-click multi-channel capture
    4. On-board software for fast acquisition and analysis, including confluency measurements and automated cell counting
    5. Access to Thermo Fisher Cloud imaging app

Consumables

  • Media (DMEM /RPMI/ PBS /HBSS /M199 /DPBS /SF 900
  • Sera ( FBS, FCS, Horse Serum, Human Serum)
  • Cell Dissociation Reagents (Trypsine /TripLE Express / TripLE select / Accutase
  • Antibodies
  • Annexin PI / MTT
  • ROS dyes (CellTrace)
  • CFSE dye
  • Trypan Blue
  • Cell fixation Buffer

PCR Machine

Best in class PCR machines from ABI, Thermo Fisher Scientfic in 5 varients

  1. Mini Amp
  2. Mini Amp Plus
  3. Simpli Amp
  4. Veriti
  5. Proflex

These can meet the requirement of Colleges and Academic Institutes to high end Research Lab.
Where MiniAmp is a low budget model,The latest model Proflex 32 X 3 well block provides, flexiblity to work as 3 independent users with different protocols and different start points. Also it has simulation mode which will help mimic ramp rate of any available model in market so easy optimization.

Consumables

  • Cloning
  • Expression vectors
  • Site directed mutagenesis
  • Vectors
  • Restriction enzymes
  • Methylation kits, CRISPR-CAS9

Gel Documentation System

The E-Gel Imager System is your personal imaging system for documenting and analyzing agarose gels and E-Gel cassettes. Each E-Gel Imager system includes a sleek and compact camera hood and one or more interchangeable bases along with two powerful software programs. There are three bases to choose from: a UV Light Base, a Blue Light Base and an E-Gel® Adaptor Base. In any of the three configurations the E-Gel® Imager system provides a small and light imaging solution that utilizes a scientific grade camera.

Consumables

  • UV Tubes

Real Time PCR

Most trusted models from ABI, Thermo Fisher Scientific

  • StepOne and StepOne Plus
  • Quant Studio 3 /5
  • Quant Studio 6 / 7 Flex
  • 7500 Series

Consumables

  • Nucleic Acid Extraction kits
  • PCR plates and tubes
  •  Taqman Assays
  • C- DNA Synthesis Kit
  • Syber Green Master Mixes
  • Taqman probes

Sequencers from ABI

  • 8 Capillary , 3500 Series Genetic Analyzer
  • QSeqStudio Genetic Analyzer

Sequencing consumables

    • POP 4/ POP 6 / POP 7
    • Big Dye Direct Cycle
    • Sequencing Kit
    • Anode Buffer
    • Cathode Buffer

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Microarray

GeneAtlas System for Academic Customers from Thermo Fisher Scientific
The GeneAtlas System utilizes a particular format – the array strip, with four arrays per strip. The array strip design and the GeneAtlas System combine to provide distinct advantages:

  • Simplified hybridization – no need to create a hybridization chamber assembly
  • Robustness that protects precious samples, unlike fragile slide-based designs
  • Limited hands-on time
  • Simple array processing with common microwell-based labware

consumables

  • Cytoscan Amplification Kit
  • GeneChip 3′ IVT Pico Kit
  • GeneChi HT Pico Kit
  • GeneChip Hybridization, Wash, and Stain Kit

Laminar Air Flow

It provides a work area with aseptic/sterile conditions for the tissue culture. Laminar Air Flow has continuous displacement of air (it provides streamline flow of air) that passes through HEPA (High Efficiency Particulate Air) filter that removes the particulates from the air.
Laminar Air Flow are equipped with a UV lamp used to sterilize the shell or cabinet or the surface
Available in Horizontal and Vetical Flow in different sizes.

consumables

  • Micropipette
  • Falcon Tubes
  • Pipette Controller
  • Surface Sterilizes

Deep Freezer

We Provide -20°C an -40°C deepfreezers from Blue Star.
Our Principals are a very reputed brand in refrigeration products have wide range of offerings from 270L to 450L capacity. We have both Vertical and Crest Freezers

consumables

  • Sample Storage Vials
  • Cryo Vials for cryopreservation of samples
  • Sample storage Boxes to accommodate 0.2 ml/ 1.5ml / 2ml MCT’s and Cryo vials
  • Cryo markers and Cryo tapes
  • Stainless steel racks for organisation of boxes

CO2 Incubator

A cell culture incubator is designed to maintain a constant temperature and high humidity for the growth of tissue culture cells under a CO2 atmosphere. Typical temperature settings range from 4°C to 50°C , and CO2 concentrations run from 0.3 to 19.9%.Models: 48L, 170 litres.

Consumables

  • Liquid Nitrogen cylinders
  • Gas controller
  • Air Vent Filters

Fully Atomated Cell Imaging System

EVOS™ FL Auto 2 Imaging System fromThermo Fisher Scientific

  • Outstanding image quality and versatility with a 5-position objective turret, 4-color fluorescence and transmitted light channels, plus color and B/W cameras
  • Exceptional usability with fully automated X/Y scanning stage, autofocus, and acquisition routines
  • High-speed image acquisition coupled with multi-position well scanning. Z-stack and tile-stitch options lend power and flexibility to your data generation.
  • High-speed image acquisition coupled with multi-position well scanning. Z-stack and tile-stitch options lend power and flexibility to your data generation.
  • Powerful image analysis capabilities for cell segmentation and quantification with the optional Celleste™ Image Analysis Software

Automated Cell Counters

Countess™ II FL Automated Cell Counter from Thermo Fisher Scientific Countess II FL provides Accurate, Flexible, Convinient and fast cell counting. Depending on the application user as flexibility to choose light cubes. Alternatively if fluorescence applications are not there user can opt of Countess II model

Consumables

  • Disposable slide holder
  • Reusable slide holder
  • Light cube removal tool
  • Disposable slides

Qubit 4.0 Flourometer

The Qubit 4 Flurometer with WiFi is the latest version of the popular Qubit fluorometer designed to accurately measure DNA, RNA and Protein quantity and now also RNA integrity and quality, using the highly sensitive Qubit assay

Consumables

  • Qubit Assay Tubes
  • Qubit 4 System Verification Assay Kit
  • Qubit 1X dsDNA HS Assay Kit
  • Qubit dsDNA BR Assay Kit
  • Qubit RNA IQ Assay Kit
  • Qubit RNA HS Assay Kit
  • Qubit Protein Assay Kit

Ultra Low Freezer -86°C from BlueStar

Stainless steel interior. Painted steel panel exterior and 4 casters for easy handing

  1. The front opening lockable door comes with a handle for easy accessibility as well as a vacuum release port
  2. Adjustable stainless-steel shelves with inner insulation doors and double silicon gasket seal
  3. Two-times foaming technology with 120 mm foaming insulation as well as a VIP insulation board
  4. Comes with USB storage functions
  5. Optional additions include: chart recorder, CO2 back up system, storage racks/boxes and remote alarm system
  6. Temperature control ranges between -40°C and -86°C that can be set freely
  7. Temperature control is precise to 1°C
  8. Along with a digital temperature display, it has a keyboard lock and a password protected configuration page
  9. The freezer has a delayed start and safe stop interval between restart and being terminated
  10. Equipped with audio and video alarms, such as: High or Low Temperature alarm, Power Failure alarm
  11. Low Battery alarm, Door Open alarm, Filter Blocking alarm and System Failure alarm”

Consumables

  • Sample Storage Vials
  • Cryo Vials for cryopreservation of samples
  • Sample storage Boxes to accommodate 0.2 ml/ 1.5ml / 2ml MCT’s and Cryo vials
  • Cryo markers and Cryo tapes
  • Stainless steel racks for organisation of boxes

Cryocan / LN2 container

  1. Cryocan for Biological application
  2. Cryocan for Transport application.(These products needed for cryogenic storage of biological material at cryogenic temperature right from semen to stem cells both in vapour & liquid nitrogen phase with focus for long term Cryogenic preservation of the biological samples and also, providing turn-key Cryobank management systems with full automated controls)

Consumables

  • Cryo Gloves
  • Cryo Labels
  • Cryovials
  • Liquid Nitrogen Withdrawal Device
  • CryoCane
  • Cryosleeve
  • Cryomarker