Cell Culture Based Research
We can grow either adherent or suspension cells as per customer specifications. Small- or large-scale cultures are carried out in cell culture flasks. The following cell culture services are available
- Adherent cell culture service
- Suspension cell culture service
- Primary cell line derivation service
- Small suspension culture or frozen cells
After culturing following cell-based assays are provided as service –
| Analysis of cell cycle by flow cytometry |
| Analysis of apoptosis by flow cytometry (Annexin-PI) method |
| Analysis of mitochondrial potential by flow cytometry |
| Analysis of Cell membrane potential by flow cytometry |
| Analysis of cell proliferation by CFSE staining (Flow cytometry) |
| Analysis of cancer cell surface markers |
| Analysis of cancer cell intracellular proteins by flow cytometry |
| Analysis of cancer stem cells by flow cytometry from different origins of cancers (Aldefluor, surface markers and intracellular proteins) |
| Culture and analysis of different normal as well as cancer cell lines for various purposes from Mice and humans |
| Scratch and migration assay for analysis of metstasis for different cancers |
| Multiplexing of cytokines by flow cytometry (CBA), same can be applied for growth factors, chemokines, Inflammatory molecules, bone metabolism molecules. |
| MicroRNA multiplexing by flow cytometry |
| Invitro differentiation of THP1 cells into macrophages |
| Invitro differentiation of Macrophages into osteoclasts |
| Analysis of Drosophila cells by flow cytometry |
| Detection of host cell infection by flow cytometry for protozoan pathogens |
| Magnetic cell sorting of CD4 T cells |
| Invitro differentiation of pancreatic ductal cells ito Beta cells |
| Magnetic cell sorting of regulatory T cells |
| Human and Mice T cell analysis |
| Human and mice B cell analysis |
| Human regulatory T cell analysis |
| Invitro immunomodulatory studies for herbal extracts and chemicals |
| Invitro immunomodulatory studies for peptides as the part of vaccine trials |
| Analysis of virus-infected GFP cells |
| Analysis of antigen-specific immune cells isolated from Lymph node and spleen after booster vaccination |
| Reactive oxygen species induction analysis |
| Analysis of cardioprotective effects of herbal extracts |
| Analysis of mice and human macrophages |
| Epithelial-mesenchymal transition (EMT) |