Cell Biology

The specific requirements of a cell culture laboratory depend mainly on the type of research conducted; for example, the needs of mammalian cell culture laboratory specializing in cancer research is quite different from that of an insect cell culture laboratory that focuses on protein expression.  However, all cell culture laboratories have the common requirement of being free from pathogenic microorganisms (i.e., asepsis), and share some of the same basic equipment that is essential for culturing cells.

This section lists the equipment and supplies common to most cell culture laboratories, as well as beneficial equipment that allows the work to be performed more efficiently or accurately, or permits wider range of assays and analyses.

Work Flow

  1. Tissue Disintegration & Single Cell Suspension Preparation
  2. Culturing of Cells
  3. Analysing The Effect of Treatment on Cells
  4. Characterization Of Cells, CTCs, CSCs

Cell Culture Based Research

Instrument Details

Laminar Air Flow

It provides a work area with aseptic/sterile conditions for the tissue culture. Laminar Air Flow has continuous displacement of air (it provides streamline flow of air) that passes through HEPA (High Efficiency Particulate Air) filter that removes the particulates from the air.
Laminar Air Flow are equipped with a UV lamp used to sterilize the shell or cabinet or the surface
Available in Horizontal and Vertical Flow in different sizes.

Consumables

  • Culture plates (6/12/24/ 48/96 well )
  • Cell culture flasks (T 25/75/225/525),
  • Serological Pipettes
  • Media (DMEM /RPMI/ PBS /HBSS /M199 /DPBS /SF 900)
  • Growth factors and Serums
  • Antbiotics and Antimycotics
  • Cell Strainers
  • Syringe Filters

Inverted Microscope

Class II, Type A2 Biological Safety Cabinets offer personnel, product and environmental protection to obtain optimum control over product quality while reducing the potential for exposure of both product and personnel to airborne biological or particulate chemical agents in low to moderate risk-hazard research and drug preparation or product operations.

Consumables

  • Culture plates (6/12/24/ 48/96 well )
  • Serological Pipettes
  • Growth factors and Serums
  •  Cell Strainers
  • Growth factors and Serums
  • Media (DMEM /RPMI/ PBS /HBSS /M199 /DPBS /SF 900)
  • Antbiotics and Antimycotics
  •  Syringe Filters

CO2 Incubator

A cell culture incubator is designed to maintain a constant temperature and high humidity for the growth of tissue culture cells under a CO2 atmosphere. Typical temperature settings range from 4°C to 50°C , and CO2 concentrations run from 0.3 to 19.9%.Models: 48L, 170 litres.

Consumables

  • Liquid Nitrogen cylinders
  • Gas controller
  • Air Vent Filters

Inverted Microscope

EVOS M5000 Imaging System
Key Advantages

      1. Easy installation; no maintenance, assembly, alignment or calibration
      2. User defined four-color fluorescence, transmitted light and color applications
      3. Autofocus, Z-stack capability, time-lapse imaging and single-click multi-channel capture
      4. On-board software for fast acquisition and analysis, including confluency measurements and automated cell counting
      5. Access to Thermo Fisher Cloud imaging app

Consumables

      • Media (DMEM /RPMI/ PBS /HBSS /M199 /DPBS /SF 900
      • Sera ( FBS, FCS, Horse Serum, Human Serum)
      • Cell Dissociation Reagents (Trypsine /TripLE Express / TripLE select / Accutase
      • Antibodies
      • Annexin PI / MTT
      • ROS dyes (CellTrace)
      • CFSE dye
      • Trypan Blue
      • Cell fixation Buffer
      • Chamber Slides / Heamocytometer
      • 6/12/24/ 48/96 well tissue culture treated plates
      • Tissue culture flasks T-25/T75/T275
      • Serological Pipettes 5ml/ 10ml
      • Cell scraper

Inverted Microscope

EVOS M5000 Imaging System
Key Advantages

    1. Easy installation; no maintenance, assembly, alignment or calibration
    2. User defined four-color fluorescence, transmitted light and color applications
    3. Autofocus, Z-stack capability, time-lapse imaging and single-click multi-channel capture
    4. On-board software for fast acquisition and analysis, including confluency measurements and automated cell counting
    5. Access to Thermo Fisher Cloud imaging app

Consumables

  • Media (DMEM /RPMI/ PBS /HBSS /M199 /DPBS /SF 900
  • Sera ( FBS, FCS, Horse Serum, Human Serum)
  • Cell Dissociation Reagents (Trypsine /TripLE Express / TripLE select / Accutase
  • Antibodies
  • Annexin PI / MTT
  • ROS dyes (CellTrace)
  • CFSE dye
  • Trypan Blue
  • Cell fixation Buffer
  • Chamber Slides / Heamocytometer
  • 6/12/24/ 48/96 well tissue culture treated plates
  • Tissue culture flasks T-25/T75/T275
  • Serological Pipettes 5ml/ 10ml
  • Cell scraper

Automated Cell Counters

Countess™ II FL Automated Cell Counter from Thermo Fisher Scientific Countess II FL provides Accurate, Flexible, Convinient and fast cell counting.
Depending on the application user as flexibility to choose light cubes. Alternatively if fluorescence applications are not there user can opt of Countess II model

Consumables

  • Disposable slide holder
  • Reusable slide holder
  • Light cube removal tool
  • Disposable slides

Flow Cytometer/Magnetic cell sorting system

Attune NxT Acoustic Focusing Cytometer, which is an easy-to-use instrument designed to detect cell size and granularity, as well as fluorescence expression of labeled antibodies, proteins, and functional reagents. The innovative design of the instrument results in precise data and acquisition speeds up to 10X faster than other flow cytometers. The design also eliminates lengthy sample preparation procedures, reduces the time to analyze a file, allows small or limited samples to be analyzed, and minimizes variability in measurements, leading to better resolution of populations. It is ideal for immunophenotyping and signaling studies, cell cycle analysis, detection of rare events, stem cell analysis, cancer and apoptosis studies, and microbiological assays

Consumables

  • DNA staining dye for ploidy level detection
  • Antibodies for tumor cells CTCs, CSCs, Aldiflour Assay, Taqman Assay
  • Calibration Beads
  • Focussing fluid
  • Wash Solution
  • Shut down solutions
  • Media (DMEM /RPMI/ PBS /HBSS /M199 /DPBS /SF 900
  • Sera ( FBS, FCS, Horse Serum, Human Serum)
  • Cell Dissociation Reagents (Trypsine /TripLE Express / TripLE select / Accutase
  • Antibodies
  • Annexin PI / MTT
  • ROS dyes (CellTrace)
  • Cell Proliferation dye( CFSE / Blue dye )
  • Trypan Blue
  • Cell fixation Buffer

Cryocan / LN2 container

  1. Cryocan for Biological application
  2. Cryocan for Transport application.
    (These products needed for cryogenic storage of biological material at cryogenic temperature right from semen to stem cells both in vapour & liquid nitrogen phase with focus for long term Cryogenic preservation of the biological samples and also, providing turn-key Cryobank management systems with full automated controls)

Consumables

  • Cryo Gloves
  • Cryo Labels
  • Cryovials
  • Liquid Nitrogen Withdrawal Device
  • Cryo Tags
  • Cryo sleeve
  • Cryo marker